General Cell Collection Detail

General Cell Collection: Bob

Catalogue No.:	10021102
Cell Line Name:	Bob
Keywords:	Human prostate cancer spontaneously immortalized, serum-free
Cell Line Description:	First spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype.  This cell line is cultured in serum-free culture medium, a derivative of Bob is also available i.e. SerBob, ECACC catalogue number 10021101, which has been adapted to medium containing serum.  SerBob is more differentiated and invasive than Bob.
Species:	Human
Tissue:	Prostate
Morphology:	Cobblestone
Growth Mode:	Adherent
Subculture Routine:	Split sub-confluent cultures (70-80%) 1:5 to 1:20  i.e. seeding at 2-4 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells must be centrifuged at 400 x g for 5 minutes to remove the trypsin at each subculture. Cells are cryopreserved in 45% conditioned media: 45% fresh media: 10% DMSO. At confluence 5-7 x 104 cells/cm² can be expected.
Culture Medium:	Keratinocyte-SFM (Invitrogen, catalogue no 37010-022, supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor,  2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.
Karyotype:	4n (81 to 94)
Products:	10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour.
Receptors:	CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR
Depositor:	Drs David Hudson & Sian Rizzo, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, UK
Country:	UK
References:	Attard G et al., (2009) A novel, spontaneously immortalized, human prostate cancer cell line, Bob, offers a unique model for pre-clinical prostate cancer studies. Prostate 69: 1507-1520 PMID: 19544327.
Patents:	None Specified By Depositor
Research Council Deposit:	No
Release Conditions:	No

The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.