General Cell Collection Detail
General Cell Collection: MDCK
| Catalogue No.: | 85011435 |
|---|---|
| Cell Line Name: | MDCK |
| Keywords: | Canine Cocker Spaniel kidney |
| Cell Line Description: | Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2). |
| Species: | Dog |
| Tissue: | kidney |
| Morphology: | Epithelial-like |
| Growth Mode: | Adherent |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA. |
| Culture Medium: | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Karyotype: | 2n = 78 |
| Depositor: | Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford |
| Country: | UK |
| References: | Proc Soc Exp Biol Med 1958;58:574; Proc Soc Exp Biol Med 1966;122:931 |
| Additional Bibliography: | Not specified |
| Patents: | None specified by Depositor |
| Research Council Deposit: | No |
| Release Conditions: | No |
The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
