General Cell Collection Detail
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General Cell Collection: A549
|Cell Line Name:||A549|
|Keywords:||Human Caucasian lung carcinoma|
|Cell Line Description:||Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.|
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.|
|Culture Medium:||Ham's F12K or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Products:||Lecithin, High amounts of de-saturated fatty acids.|
|Depositor:||Obtained from ATCC|
|References:||Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP. 1973 In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.J Natl Cancer Inst. 51(5):1417-23. PMID: 4357758|
|Additional Bibliography:||Lieber M, Smith B, Szakal A, Nelson-Rees W, Todaro G 1976 A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer. 15;17(1):62-70. PMID: 175022|
|Patents:||None specified by Depositor|
|Research Council Deposit:||No|
The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.