General Cell Collection Detail

General Cell Collection: A549

Catalogue No.:	86012804
Cell Line Name:	A549
Keywords:	Human Caucasian lung carcinoma
Cell Line Description:	Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.
Species:	Human
Tissue:	lung
Morphology:	Epithelial
Growth Mode:	Adherent
DNA Profile:	STR-PCR Data: Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11 D16S539: 11,12 D5S818: 11 D7S820: 8,11 THO1: 8,9.3 TPOX: 8,11 vWA: 14
Subculture Routine:	Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Culture Medium:	Ham's F12K or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype:	Hypotriploid
Products:	Lecithin, High amounts of de-saturated fatty acids.
Depositor:	Obtained from ATCC
Originator:	No
Country:	USA
References:	Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP. 1973 In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.J Natl Cancer Inst. 51(5):1417-23. PMID: 4357758
Additional Bibliography:	Lieber M, Smith B, Szakal A, Nelson-Rees W, Todaro G 1976 A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer. 15;17(1):62-70. PMID: 175022
Patents:	None specified by Depositor
Research Council Deposit:	No
Release Conditions:	No

The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.