General Cell Collection Detail

General Cell Collection: C2C12

Catalogue No.:	91031101
Cell Line Name:	C2C12
Keywords:	Mouse C3H muscle myoblast
Cell Line Description:	Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.
Species:	Mouse
Tissue:	muscle
Morphology:	Myoblast
Growth Mode:	Adherent
Subculture Routine:	Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Do not allow cells to reach confluence.
Culture Medium:	DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS).
Karyotype:	Not specified
Depositor:	Obtained from ATCC
Originator:	No
Country:	USA
References:	Nature 1977;270:725, Science 1985;230;758-766, J. Cell Biol. 1994; 127:1755-1766[published erratum appears in J Cell Biol 1995 Feb;128(4):following 713], J.Virol. 1997;71:169-178, Proc. Natl. Acad.Sci. USA 1996;93:14082-14087, J. Biol. Chem. 1996;271:1386
Additional Bibliography:	(1)J Anim Sci; 1996 Jun: 74(6): 1265-73
Patents:	None specified by Depositor
Research Council Deposit:	No
Release Conditions:	No

The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.