General Cell Collection Detail
Cell Line Authentication & Quality Control Information
Print datasheetGeneral Cell Collection: A2780ADR
| Catalogue No.: | 93112520 |
|---|---|
| Cell Line Name: | A2780ADR |
| Keywords: | Human Caucasian ovarian carcinoma |
| Cell Line Description: | The adriamycin-resistant cell line A2780ADR has been developed by exposure of the parent A2780 cell line (ECACC catalogue no. 93112519) to adriamycin. A2780ADR is cross-resistant to melphalan and vinblastine. To retain resistance adriamycin has to be added to the media. The cells grow as a monolayer and in suspension in spinner cultures and are tumourigenic in immune deficient mice. Together with the cisplatin-resistant variant A2780cis these lines only differ in their exposure to a single drug and should facilitate the search for molecular changes responsible for the expression of pleiotropic drug resistance in human ovarian cancer. |
| Species: | Human |
| Tissue: | ovary |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| DNA Profile: |
STR-PCR Data: Amelogenin: X |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established. |
| Culture Medium: | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week. |
| Karyotype: | Not specified |
| Depositor: | Dr T H Ward, Cell Culture Unit, Patterson Laboratories, Christie Hospital, Manchester |
| Originator: | No |
| Country: | UK |
| References: | Proc Amer Assoc Cancer Res 1984;25:336; Seminars in Oncology 1984;11:285 |
| Additional Bibliography: | Not specified |
| Patents: | None specified by Depositor |
| Research Council Deposit: | No |
| Release Conditions: | No |
The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Available Formats
Frozen
Growing
DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)