General Cell Collection Detail
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General Cell Collection: NCI-H69/CPR
|Cell Line Name:||NCI-H69/CPR|
|Keywords:||Human small cell lung cancer, drug-resistant|
|Cell Line Description:||NCI-H69/CPR is a drug resistant subline of NCI-H69 (ECACC catalogue no. 91091802). Drug resistance was developed by addition of a stepwise increase in cisplatin to the growth medium of the parental line. The cell line exhibits a 5-fold resistance to cisplatin and is cross resistant to melphalan. A significant change in cellular glutathione content or sensitivity to cadmium chloride (as indicator of metallothionein content) was not detected, but changes in glutathione-S-transferase activity were seen. Cisplatin accumulation was unchanged compared with the parent line. It is recommended to culture the cells without drugs after resuscitation until the first passage.|
|Growth Mode:||Aggregates in suspension|
|Subculture Routine:||Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37ºC; resuscitate in drug-free medium until first passage.|
|Culture Medium:||RPMI 1640 + 2mM Glutamine + 0.4µg/ml cisplatin + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Dr P Twentyman, Dr. K. Wright, UKCCR, PO Box 123, Lincolns Inn Fields, London WC2A 3PX|
|References:||Br J Cancer 1992;65:684, Cancer Res 1992;52:5674|
|Additional Bibliography:||Not specified|
|Patents:||None specified by Depositor|
|Research Council Deposit:||Yes|
The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.