Hybridoma Detail
Hybridoma Collection: AFRC MAC 61.6
| Catalogue No.: | 01010804 |
|---|---|
| Cell Line Name: | AFRC MAC 61.6 |
| Reactivity: | Anti- abscisic acid, plant growth hormone |
| Antibody Isotype: | IgG2C |
| Myeloma: | YB2/3.0 Ag20 |
| Immunological Donor: | F344 Rat spleen |
| Immunogen: | S-cis, trans-abscisic acid - 4(1)- p-amino-benzoyl-hydrazone-KLH conjugate |
| Cell Line Description: | This cell line is the result of recloning of AFRC MAC 61. The antibody is directed against the plant growth hormone cis, trans-abscisic acid. The antibody production rate of this hybridoma has been tested at ECACC. It must be noted that this hybridoma produces less than 2µg/ml of unpurified antibody. |
| Sub Culture Routine: | Maintain cultures between 3-9 x 100,000 cell/ml; 5%CO2; 37°C. Require mouse macrophage feeder layers upon resuscitation |
| Culture Medium: | DMEM + 2mM glutamine + 10% FBS |
| Depositor: | Dr GW Butcher, Babraham Institute, Cambridge |
| Originator: | Yes |
| Country: | UK |
| References: | Analytical Biochemistry 1985;151: 389-399 |
| Research Council Deposit: | No |
The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
