Hybridoma Detail
Hybridoma Collection: AFRC MAC 252
| Catalogue No.: | 01071008 |
|---|---|
| Cell Line Name: | AFRC MAC 252 |
| Reactivity: | Anti- abscisic acid, plant growth hormone |
| Antibody Isotype: | IgG2a |
| Fusion Species: | Rat x Rat Hybridoma |
| Myeloma: | Y3 Ag 1.2.3 |
| Immunological Donor: | LOU rat spleen |
| Immunogen: | S-cis, trans-abscisic acid - 4(1) - p-amino-benzoyl hydrazone-KLH conjugate |
| Transfected DNA: | Not Applicable |
| Cell Line Description: | This hybridoma is a subclone of AFRC MAC 60. The antibody is directed against the plant growth substance cis, trans-abscisic acid. The rate of antibody production has been tested at ECACC. It must be noted that this hybridoma produces less than 2 µg/ml of purified antibody. |
| Sub Culture Routine: | Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2, 37°C. Require mouse macrophage feeder layers upon resuscitation. |
| Culture Medium: | DMEM + 2mMGlutamine + 10% FBS |
| Depositor: | Dr. GW Butcher, Babraham Institute, Cambridge |
| Originator: | Yes |
| Country: | UK |
| References: | Analytical Biochemistry 1985;151: 389-399 |
| Patent: | None Specified by Depositor |
| Research Council Deposit: | Yes |
| Release Conditions: | No |
The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
