Hybridoma Detail
Hybridoma Collection: 2G2B(AAT) Clone 4D4
| Catalogue No.: | 02043016 |
|---|---|
| Cell Line Name: | 2G2B(AAT) Clone 4D4 |
| Reactivity: | Anti- alpha1-antitrypsin (also known as alpha1-AT or AAT) |
| Antibody Isotype: | IgG2b |
| Fusion Species: | Mouse x Mouse Hybridoma |
| Myeloma: | Sp2/0 |
| Immunological Donor: | BALB/c mouse spleen |
| Immunogen: | Various peptides between 9 and 11 amino acids attached to PPD via a terminal cysteine residue |
| Transfected DNA: | Not Applicable |
| Cell Line Description: | The antibody is directed against alpha1-antitrypsin (also known as alpha1-AT or AAT). |
| Application: | ELISA, lateral flow tests, western blot analysis |
| Sub Culture Routine: | Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Split ratio: 1:2 |
| Culture Medium: | RPMI 1640 + 2mM glutamine + 15% Foetal Bovine Serum (FBS) |
| Depositor: | Dr. Koen Verhoef, MRC Technology, 20 Park Crescent, London, UK |
| Originator: | No |
| Country: | UK |
| References: | None specified by depositor |
| Patent: | None specified by depositor |
| Research Council Deposit: | No |
| Release Conditions: | No |
The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
