Hybridoma Detail
Hybridoma Collection: 2D12
| Catalogue No.: | 91051509 |
|---|---|
| Cell Line Name: | 2D12 |
| Reactivity: | Anti- yellow fever virus |
| Antibody Isotype: | IgG2a, kappa |
| Fusion Species: | Mouse x Mouse Hybridoma |
| Myeloma: | P3X63Ag8.653 |
| Immunological Donor: | BALB/c mouse spleen |
| Immunogen: | Yellow fever virus, strain 17D |
| Transfected DNA: | Not Applicable |
| Cell Line Description: | The antibody is directed against the envelope protein of the wild type and vaccine strains of yellow fever virus. It does not cross react with other flaviviruses. |
| Sub Culture Routine: | Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. |
| Culture Medium: | RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-mercaptoethanol (2ME) + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) |
| Depositor: | Obtained from ATCC |
| Originator: | No |
| Country: | USA |
| References: | Virology 1983;125:8; Am J Trop Med Hyg 1984;33:695 |
| Patent: | None Specified by Depositor |
| Research Council Deposit: | No |
| Release Conditions: | No |
The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
