DNA Detail
View product documentsDNA Panels: HRC-2 DNA Panel
| Product Name: | HRC-2 DNA Panel |
|---|---|
| Catalogue No.: | 06041302 |
| Description: | Human Random Control DNA Panel 2 (HRC-2) |
| Product Type: | Human Random Control (HRC) DNA is extracted from lymphoblastoid cell lines derived by Epstein Barr Virus (EBV) transformation of peripheral blood lymphocytes from fresh, single donor blood samples. The donors are all UK Caucasian and are characterised by gender and age at venesection, this information where known is detailed in the datasheet for the panel. All donors have given written, informed consent for their blood to be used for research purposes. As the DNA is derived from immortal single donor cell lines this is a renewable supply of DNA that can be reproduced batch to batch. |
| How To Use: | ECACC Human Random Control (HRC) |
| Format: | 96 x 2µg array (8 x 12 well format), each well represents an individual sample |
| Concentration: | 100ng DNA/µl. The DNA is dissolved in 10mM Tris (pH8) 1mM EDTA to preserve the quality of the DNA. |
| Storage Conditions: | ECACC DNA arrays are supplied frozen to preserve quality and also to facilitate handling. On receipt, panels should be stored at -80°C. The DNA array presentation is intended for single use although, with care, repeat use is possible. After thawing, panel can be stored at 2-8 °C for 6 months, provided precautions are taken to prevent fluid loss by evaporation.Repeated freeze-thawing cycles can damage the DNA and should be avoided. |
| Quality Control Information: | The DNA is extracted and purified using standard techniques that yield high molecular weight material, as verified by agarose gel electrophoresis. The composition of each panel is completely defined and standardised so that each product lot will be identical. The identity of each product lot is checked using STR DNA profiling. Consequently ECACC HRC DNA Panels can be used as Reference Standard material as an aid to routine quality control in the laboratory. |
| Source: | DNA is derived from cell lines in the Human Random Control subgroup of the Human Genetic Collection |
| Special Release Conditions: | Standard Terms & Conditions of Supply apply. |
| Datasheet: | Click here |
The HPA Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by HPA Culture Collections are accurate, HPA Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and HPA Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by HPA Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
