RNA from Cell Lines
ECACC offers high quality RNA prepared from cell lines; cell lines in our catalogues and customer's own cell lines.
For ECACC cell lines RNA is now an 'add to cart' format with price and availability displayed on each cell line detail webpage; visit our cell line catalogue items to view. To request a quote for RNA prepared from your own cell lines or more information please complete the form below.
All RNA is quantified using a Nanodrop N1000 spectrophotometer and normalised to a standard concentration of 100ng/µl. As part of our routine Quality Assurance, a proportion of each batch of RNA is tested for purity using the nucleic acid 260:280 and 260:230 absorbance ratios. RNA size and integrity are measured using agarose gel electrophoresis.
Typical Quality scores for ECACC prepared RNA:
A260/280: 2.05
A260/230: 2.1
RIN score: 9.875
18S:28S ratio: 2.3
The capability to generate high quality RNA also enables the ability to generate high quality cDNA. ECACC will soon be making a range of cDNA panels available from its unique cell lines. cDNA provides a rapid, cost effective reagent to screen cell lines for particular expression characterisitics in order to select cell lines of most relevance to take forward for more in depth analyses e.g. time consuming pharmacogenetic analysis.
If you are interested in obtaining cDNA from cell lines, please contact us using the enquiry form below.
RNA and cDNA Application Note Poster: Proof of Principle - Application of ECACC RNA and cDNA in Cell Line Receptor Characterisation
To request a quote for the preparation of RNA from your own cell lines (prices for RNA from ECACC cell lines are displayed on the cell line detail webpages) or more information – please complete and submit the form below:
