DNA Profiling of Cell Lines

DNA profiling of cell lines is a powerful tool used to demonstrate continuity between master and working cell banks, or between source material and derived cell lines, and to identify contamination of cell lines. ECACC uses Microsatellite Genotyping for its Cell Line Verification Service:

Microsatellite Genotyping

A quick and powerful tool for the verification of human cell lines. ECACC uses the Applied Biosciences Identifiler Plus® system for DNA profiling. This is the same system used by the forensic science services and amplifies 15 highly informative short tandem repeat (STR) loci plus the gender marker Amelogenin. These afford, on average, a 1 in 5 trillion probability of identity, producing a unique profile that can be compared with those held on ECACC's database.