Frozen Cells for DNA and RNA Extraction
Frozen cell pellets can be produced from any of the cell lines available in ECACC's catalogues* in addition to customer's own deposits. Cell lines can be expanded to any number of cells, or volume of culture specified; however, for convenience the majority of Genetic Support Service customers choose, frozen lymphoblastoid cell pellets prepared from four standard culture volumes (see Table 1).
Cell pellets can be utilised for DNA and/or RNA extraction and our protocols have been specifically designed to suit the differing requirements, in terms of point of cell harvest, reagents used, and freeze methods.
Table 1: Standard cell culture volumes & approximate number of lymphoblastoid cells available as frozen cell pellets from ECACC
| Culture Volume | Approx. No. of Cells |
|---|---|
| 30-40ml | 1-2 x 107 |
| 50ml | 3-4 x 107 |
| 120ml | 1 x 108 |
| 500ml | 3-4 x 108 |
ECACC also offers a comprehensive Contract Cell Culture service, combining a wide range of cell culture systems with extensive cell culture expertise to address more specialised customer requirements.
Contact us to discuss your requirements in more detail or for a specific quotation.
*Excludes Primary Cells
