Cell Culture Reagents
The following list of products, available from Sigma-Aldrich, is provided to aid acquisition of reagents commonly used by ECACC for the culture of cell lines.
| Sigma Cat No. | Product | Description |
| M6250 | 2ME | 2 – Mercaptoethanol |
| M4526 | ALPHA MEM | Minimum Essential Medium Eagle’s – Alpha Modification |
| B1522 | BME | Basal Medium Eagle |
| B9285 | Budr | Bromo-deoxy Uridine |
| C5467 | CHO PF Media | CHO Protein Free Medium - Animal Component Free |
| C8919 | Collagen Type I | Collagen Type I |
| C5533 | Collagen Type IV | Collagen Type IV |
| F6636 | Coon’s F12 | Nutrient Mixture F-12 Ham Coon’s Modification |
| C6278 | CS | Calf Serum |
| D6546 | DMEM | DME, Dulbecco’s Modified Eagle’s Medium |
| D8062 | DMEM:F12 (1:1) | DMEM:Nutrient Mix F12 Ham (1:1) ratio mix |
| D2650 | DMSO | Dimethyl Sulphoxide |
| M2279 | EMEM (EBSS) | Minimum Essential Medium Eagle’s (Earle’s Balanced Salt Solution) |
| M5775 | EMEM (HBSS) | Minimum Essential Medium Eagle’s (Hank’s Balanced Salt Solution) |
| F2442 | FBS / FCS | Foetal Bovine Serum / Foetal Calf Serum (USA Origin) |
| F4135 | FBS HI | Foetal Bovine Serum – Heat Inactivated (USA Origin) |
| F4759 | Fibronectin | Fibronectin |
| F5008 | Fischer’s Medium | Fischer’s Medium |
| C2639 | Freeze Media SF | Cell Freezing Medium – Serum free |
| G9391 | Gelatin | Gelatin |
| G7513 | Glutamine | L-Glutamine |
| G5150 | Glycerol | Glycerol |
| G5154 | GMEM | GlasgowMinimum Essential Medium |
| N6013 | Ham’s F10 | Nutrient Mixture F-10 Ham’s |
| N4888 | Ham’s F12 | Nutrient Mixture F-12 Ham’s |
| H0887 | Hepes | Hepes |
| H8142 | HES | Hybridoma Enhancing Supplement |
| H1270 | HS | Horse Serum |
| I3390 | IDMEM | IMDM / Iscove’s Modified Dulbecco’s Medium |
| I3146 | ITS | Insulin – Transferrin – Selenium |
| M8028 | JMEM | Minimum Essential Medium Eagle’s – Joklik Modification |
| N3520 | Kaighn’s F12 | Nutrient Mixture F-12 Ham Kaighn’s Modification |
| L5520 | L15 | L-15 Medium (Leibovitz) |
| A5006 | L-Arginine | L-Arginine |
| L9010 | LEH | Lactalbumin Enzymatic Hydrolysate |
| M2154 | M199 | Medium 199 |
| M8403 | McCoy’s 5A | McCoy’s 5A Medium Modified |
| M6395 | MCDB 105 | MCDB 105 Medium |
| S8761 | NaHCO3 | Sodium Bicarbonate |
| S8636 | NaP | Sodium Pyruvate |
| N4637 | NBCS | New Born Calf Serum |
| N1140 | NCTC 109 | NCTC 109 Medium |
| M7145 | NEAA | Non Essential Amino Acids |
| D8537 | PBS | Phosphate Buffered Saline |
| P8139 | PMA | Phorbol Myristate Acetate |
| R0883 | RPMI 1640 | Roswell Park Memorial Institute |
| S9895 | SDM | Schneider’s Drosophila Medium |
| M8167 | SMEM | Minimum Essential Medium Eagle’s - Spinner Modification |
| S2513 | Swimms S-77 | Swimm’s S-77 Medium |
| T8159 | TPB | Tryptose Phosphate Broth |
| T8154 | Trypan Blue | Trypan Blue |
| T4424 | Trypsin | Trypsin |
| T6522 | Trypsin Inhibitor | (Soybean) Trypsin Inhibitor |
| T4049 | Trypsin/EDTA | 0.25% Trypsin / EDTA |
| M6895 | VITS (MEM) | MEM Vitamins |
| R7256 | VITS (RPMI) | RPMI Vitamins |
| W1625 | WMB 752/1 | Waymouth Medium MB 752/1 |
